Luciferase-based transgenic recombination assay is more sensitive than beta-glucoronidase-based.

@article{Ilnytskyy2004LuciferasebasedTR,
  title={Luciferase-based transgenic recombination assay is more sensitive than beta-glucoronidase-based.},
  author={Yaroslav Ilnytskyy and Alexander Boyko and Igor Kovalchuk},
  journal={Mutation research},
  year={2004},
  volume={559 1-2},
  pages={
          189-97
        }
}
Study of the DNA repair and genome stability in plants is directly dependent on the availability of an easy, inexpensive, and reliable assay. Marker gene-based homologous recombination (HR) assays were introduced more than a decade ago and have been intensively used ever since. Here, we compared several transgenic Arabidopsis and tobacco lines that carried in their genome the luciferase (LUC) or the beta-glucoronidase (uidA or GUS) substrates for HR. The average recombination frequency detected… CONTINUE READING

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