Lowering the isoelectric point of the Fv portion of recombinant immunotoxins leads to decreased nonspecific animal toxicity without affecting antitumor activity.

@article{Onda2001LoweringTI,
  title={Lowering the isoelectric point of the Fv portion of recombinant immunotoxins leads to decreased nonspecific animal toxicity without affecting antitumor activity.},
  author={Masanori Onda and Satoshi Nagata and Yasuo M Tsutsumi and James J. Vincent and Qiu-yan Wang and Robert J. Kreitman and B Lee and Ira Pastan},
  journal={Cancer research},
  year={2001},
  volume={61 13},
  pages={5070-7}
}
Recombinant immunotoxins are genetically engineered proteins in which the Fv portion of an antibody is fused to a toxin. Our laboratory uses a 38-kDa form of Pseudomonas exotoxin A termed PE38 for this purpose. Clinical studies with immunotoxins targeting CD25 and CD22 have shown that dose-limiting side effects are attributable to liver damage and other inflammatory toxicities. We recently showed that mutating exposed surface neutral residues to acidic residues in the framework region of the Fv… CONTINUE READING