• DOI:10.1098/rspb.1957.0068
Corpus ID: 33582102

Low-temperature storage of mammalian spermatozoa

@article{Polge1957LowtemperatureSO,
  title={Low-temperature storage of mammalian spermatozoa},
  author={Ernest John Christopher Polge},
  journal={Proceedings of the Royal Society of London. Series B - Biological Sciences},
  year={1957},
  volume={147},
  pages={498 - 508}
}

Ernest John Christopher Polge

Published 1957

Biology, Medicine

Proceedings of the Royal Society of London. Series B - Biological Sciences

Experiments in this and other countries on the preservation of spermatozoa at very low temperatures have shown that no mammalian spermatozoa so far examined survive freezing when they are cooled ultra-rapidly from temperatures above freezing point to temperatures of — 79° C or below. Slow cooling and the addition of glycerol to the media in which the spermatozoa are suspended, however, permits survival of the spermatozoa of many species. In different animals, there are marked variations in the… CONTINUE READING

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