Low-temperature storage of mammalian spermatozoa

@article{Polge1957LowtemperatureSO,
  title={Low-temperature storage of mammalian spermatozoa},
  author={Ernest John Christopher Polge},
  journal={Proceedings of the Royal Society of London. Series B - Biological Sciences},
  year={1957},
  volume={147},
  pages={498 - 508}
}
  • E. Polge
  • Published 17 December 1957
  • Biology, Medicine
  • Proceedings of the Royal Society of London. Series B - Biological Sciences
Experiments in this and other countries on the preservation of spermatozoa at very low temperatures have shown that no mammalian spermatozoa so far examined survive freezing when they are cooled ultra-rapidly from temperatures above freezing point to temperatures of — 79° C or below. Slow cooling and the addition of glycerol to the media in which the spermatozoa are suspended, however, permits survival of the spermatozoa of many species. In different animals, there are marked variations in the… 
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References

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Biological applications of freezing and drying
TLDR
This chapter discusses biological applications of freezing and drying and their applications in the context of desorption, as well as some of the examples from the literature.
Life and death at low temperatures