Low-temperature storage of mammalian spermatozoa

  title={Low-temperature storage of mammalian spermatozoa},
  author={Ernest John Christopher Polge},
  journal={Proceedings of the Royal Society of London. Series B - Biological Sciences},
  pages={498 - 508}
  • E. Polge
  • Published 17 December 1957
  • Biology, Medicine
  • Proceedings of the Royal Society of London. Series B - Biological Sciences
Experiments in this and other countries on the preservation of spermatozoa at very low temperatures have shown that no mammalian spermatozoa so far examined survive freezing when they are cooled ultra-rapidly from temperatures above freezing point to temperatures of — 79° C or below. Slow cooling and the addition of glycerol to the media in which the spermatozoa are suspended, however, permits survival of the spermatozoa of many species. In different animals, there are marked variations in the… 
Effect of Cooling, Freezing and Thawing Rates and Storage Conditions on Preservation of Human Spermatozoa
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Cryopreservation and transport of mouse spermatozoa at -79°C
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The influence of diluents and processing time after ejaculation on the revival of deep-frozen bull spermatozoa
  • I. Martin
  • Biology
    The Journal of Agricultural Science
  • 1963
The investigation of the equilibration phenomenon showed that an improvement in survival after deep-freezing occurred if diluted semen was stored for up to 12 hr. after dilution, but this was not dependent on the presence of glycerol in the diluent during this ageing process.
Different Cooling Rate for Cryopreservation of Semen in Various Livestock Species: A Review
It has long been known that cooling bovine, ovine, caprine, swine stallion semen too rapidly between 30°C and 0°C induces a lethal stress in sperms proportional to cooling rate and temperature range
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The effects of freezing procedures, osmolarity, trehalose, and sucrose on survival of bull sperm in whole milk and egg yolk-Tris, used worldwide, were studied and provide a basis for further investigating simple freezing systems that might be more effective in preserving bull sperm than those currently available.
Survival Rates of Rapidly Frozen Bovine Spermatozoa
Summary Subsamples of semen from each of two ejaculates from each of 12 bulls were extended with a yolk-citrate-glycerol extender, equilibrated 5 to 6 hr. at 5° C., and placed directly in a freezing


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