Low-temperature storage of mammalian spermatozoa
@article{Polge1957LowtemperatureSO, title={Low-temperature storage of mammalian spermatozoa}, author={Ernest John Christopher Polge}, journal={Proceedings of the Royal Society of London. Series B - Biological Sciences}, year={1957}, volume={147}, pages={498 - 508} }
Experiments in this and other countries on the preservation of spermatozoa at very low temperatures have shown that no mammalian spermatozoa so far examined survive freezing when they are cooled ultra-rapidly from temperatures above freezing point to temperatures of — 79° C or below. Slow cooling and the addition of glycerol to the media in which the spermatozoa are suspended, however, permits survival of the spermatozoa of many species. In different animals, there are marked variations in the…
73 Citations
Effect of Cooling, Freezing and Thawing Rates and Storage Conditions on Preservation of Human Spermatozoa
- Chemistry, MedicineAndrologia
- 1984
Summary: Human spermatozoa was relatively resistant to cooling shock. However, when diluted semen was cooled faster than 10°C per minute from room temperature (RT) to 5° C and rewarmed to RT,…
Cryopreservation and transport of mouse spermatozoa at -79°C
- Biology
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It is indicated that mouse spermatozoa can survive cryopreservation in an ultra-low temperature freezer (-79 °C) for up to 4 months and transport at -79°C, and in vitro fertilization rates of frozen-thawed sperm after transport after transport were high.
The effect of cooling rate on the survival of cryopreserved bull, ram, and boar spermatozoa: a comparison of two controlled-rate cooling machines.
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The influence of diluents and processing time after ejaculation on the revival of deep-frozen bull spermatozoa
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The investigation of the equilibration phenomenon showed that an improvement in survival after deep-freezing occurred if diluted semen was stored for up to 12 hr. after dilution, but this was not dependent on the presence of glycerol in the diluent during this ageing process.
The high viscosity encountered during freezing in glycerol solutions: effects on cryopreservation.
- BiologyCryobiology
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Different Cooling Rate for Cryopreservation of Semen in Various Livestock Species: A Review
- BiologyInternational Journal of Current Microbiology and Applied Sciences
- 2018
It has long been known that cooling bovine, ovine, caprine, swine stallion semen too rapidly between 30°C and 0°C induces a lethal stress in sperms proportional to cooling rate and temperature range…
Cryopreservation Techniques for Ram Sperm
- BiologyVeterinary medicine international
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The current study compiled and compared the research on the impact of different cryopreservation procedures, cooling rates, equilibration time, and thawing protocols on post-thaw ram semen quality.
Survival of bull sperm frozen at different rates in media varying in osmolarity.
- Biology, MedicineCryobiology
- 1998
The effects of freezing procedures, osmolarity, trehalose, and sucrose on survival of bull sperm in whole milk and egg yolk-Tris, used worldwide, were studied and provide a basis for further investigating simple freezing systems that might be more effective in preserving bull sperm than those currently available.
Survival Rates of Rapidly Frozen Bovine Spermatozoa
- Chemistry
- 1960
Summary Subsamples of semen from each of two ejaculates from each of 12 bulls were extended with a yolk-citrate-glycerol extender, equilibrated 5 to 6 hr. at 5° C., and placed directly in a freezing…
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