Low-temperature-induced Golgi tubules are transient membranes enriched in molecules regulating intra-Golgi transport.

@article{MartnezAlonso2007LowtemperatureinducedGT,
  title={Low-temperature-induced Golgi tubules are transient membranes enriched in molecules regulating intra-Golgi transport.},
  author={Emma Mart{\'i}nez-Alonso and Jos{\'e} Antonio Camacho Ballesta and Jos{\'e} A Mart{\'i}nez-Men{\'a}rguez},
  journal={Traffic},
  year={2007},
  volume={8 4},
  pages={
          359-68
        }
}
The incubation of HeLa cells at 15 degrees C induces the formation of Golgi tubules, which contain glycosylation enzymes but neither cargo nor matrix proteins. We now show by immunofluorescence and immunoelectron microscopy that these tubules are enriched in a specific set of SNARE and Rab proteins mediating intra-Golgi transport (Gos28, GS15 and Rab6) but excluded others involved in endoplasmic reticulum-Golgi trafficking (Sec22, membrin, Rab 1 and Rab2). In vivo experiments using cyan… CONTINUE READING
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