• DOI:10.1006/PREP.2001.1395
Corpus ID: 28715604

Low-temperature increases the yield of biologically active herring antifreeze protein in Pichia pastoris.

@article{Li2001LowtemperatureIT,
  title={Low-temperature increases the yield of biologically active herring antifreeze protein in Pichia pastoris.},
  author={Z. Li and F. Xiong and Q. Lin and M. D'anjou and A. Daugulis and D. S. Yang and C. Hew},
  journal={Protein expression and purification},
  year={2001},
  volume={21 3},
  pages={
          438-45
        }
}

Z. Li, F. Xiong, +4 authors C. Hew

Published 2001

Biology, Medicine

Protein expression and purification

Antifreeze proteins and antifreeze glycoproteins are structurally diverse molecules that share a common property in binding to ice crystals and inhibiting ice crystal growth. Type II fish antifreeze protein of Atlantic herring (Clupea harengus harengus) is unique in its requirement of Ca(2+) for antifreeze activity. In this study, we utilized the secretion vector pGAPZalpha A to express recombinant herring antifreeze protein (WT) and a fusion protein with a C-terminal six-histidine tag (WT-6H… CONTINUE READING

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