Low temperature development of winter rye leaves alters the detergent solubilization of thylakoid membranes.

@article{Griffith1986LowTD,
  title={Low temperature development of winter rye leaves alters the detergent solubilization of thylakoid membranes.},
  author={Melissa Griffith and Norman P. A. Huner and Donald B. Hayden},
  journal={Plant physiology},
  year={1986},
  volume={81 2},
  pages={
          471-7
        }
}
Thylakoids isolated from leaves of winter rye (Secale cereale L. cv Puma) grown at either 20 or 5 degrees C were extracted with the nonionic detergents Triton X-100 and octyl glucoside. Less total chlorophyll was extracted from 5 degrees C thylakoids by these detergents under all conditions, including pretreatment with cations. Thylakoids from either 20 or 5 degrees C leaves were solubilized in 0.7% Triton X-100 and centrifuged on sucrose gradients to purify the light harvesting complex (LHCII… CONTINUE READING
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