Low-resolution sequencing of Rhodobacter sphaeroides 2.4.1T: chromosome II is a true chromosome.

@article{Choudhary1997LowresolutionSO,
  title={Low-resolution sequencing of Rhodobacter sphaeroides 2.4.1T: chromosome II is a true chromosome.},
  author={M. Choudhary and C. Mackenzie and Kirsten S. Nereng and Erica Sodergren and George Weinstock and S. Kaplan},
  journal={Microbiology},
  year={1997},
  volume={143 ( Pt 10)},
  pages={3085-99}
}
The photosynthetic bacterium Rhodobacter sphaeroides 2.4.1T has two chromosomes, CI (approximately 3.0 Mb) and CII (approximately 0.9 Mb). In this study a low-redundancy sequencing strategy was adopted to analyse 23 out of 47 cosmids from an ordered CII library. The sum of the lengths of these 23 cosmid inserts was approximately 495 kb, which comprised approximately 417 kb of unique DNA. A total of 1145 sequencing runs was carried out, with each run generating 559 +/- 268 bases of sequence to… CONTINUE READING

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