Low molecular weight polyethylenimine for efficient transfection of human hematopoietic and umbilical cord blood-derived CD34+ cells.

@article{Shin2005LowMW,
  title={Low molecular weight polyethylenimine for efficient transfection of human hematopoietic and umbilical cord blood-derived CD34+ cells.},
  author={Jee-Young Shin and Dongchul Suh and Jung Mogg Kim and Han-gon Choi and Jung Ae Kim and Jung Jae Ko and Yong Bok Lee and Jin Seok Kim and Y O Oh},
  journal={Biochimica et biophysica acta},
  year={2005},
  volume={1725 3},
  pages={377-84}
}
With the emerging role of hematopoietic stem cells as potential gene and cell therapy vehicles, there is an increasing need for safe and effective nonviral gene delivery systems. Here, we report that gene transfer and transfection efficiency in human hematopoietic and cord blood CD34+ cells can be enhanced by the use of low molecular weight polyethylenimine (PEI). PEIs of various molecular weights (800-750,000) were tested, and our results showed that the uptake of plasmid DNA by hematopoietic… CONTINUE READING