Low molecular weight humic substances stimulate H+-ATPase activity of plasma membrane vesicles isolated from oat (Avena sativa L.) roots

@article{Varanini2004LowMW,
  title={Low molecular weight humic substances stimulate H+-ATPase activity of plasma membrane vesicles isolated from oat (Avena sativa L.) roots},
  author={Zeno Varanini and Roberto Pinton and Mariella De Biasi and Stefania Astolfi and Andrea Maggioni},
  journal={Plant and Soil},
  year={2004},
  volume={153},
  pages={61-69}
}
The effect of <5 KDa (low molecular weight, LMW) and >5 KDa (high molecular weight, HMW) humic fractions on transport activities of isolated plasma membrane vesicles was studied. The K+-stimulated component of the ATP-hydrolyzing activity was considerably increased by LMW humic substances at concentrations ranging from 0.075 mg org CL-1 to 1 mg org CL-1. The stimulation was still evident when the detergent Brij-35 was added in the assay mixture, indicating a direct effect of LMW humic… CONTINUE READING
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