Low molecular weight cyclin E is specific in breast cancer and is associated with mechanisms of tumor progression.

Abstract

Low molecular weight (LMW) isoforms of cyclin E are post-translationally generated in breast cancer cells and are associated with aggressive disease and poor prognosis. In this study, the specificity of LMW cyclin E to cancer cells was determined by measuring cyclin E expression in tumor and non-tumor tissue from 340 breast cancer patients. Our results reveal the LMW isoforms were detected significantly more frequently in breast tumor tissue than in adjacent non-tumor breast tissues (p < 0.0001). The biologic consequences of the LMW isoforms were studied using a non-tumorigenic mammary epithelial cell line transfected with the cyclin E isoforms and resulted in increased clonogenicity, the inability to enter quiescence in response to growth factor deprivation and genomic instability compared to the full-length cyclin E. Biochemical differences between the full-length and the LMW isoforms were also evident. Biacore analyses show that the LMW isoforms have more efficient binding to CDK2 compared to full-length cyclin E, which could account for the unique biologic consequences observed with the expression of LMW cyclin E. The LMW isoforms of cyclin E are tumor specific, and are biochemically and biologically distinct from the full-length cyclin E which could provide a novel role in breast cancer progression.

DOI: 10.4161/cc.8.7.8119

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@article{Wingate2009LowMW, title={Low molecular weight cyclin E is specific in breast cancer and is associated with mechanisms of tumor progression.}, author={Hannah Wingate and Agnes Puskas and Mylinh Duong and Tuyen Bui and Dana Richardson and Yanna Liu and Susan L Tucker and Carolyn Van Pelt and Laurent Meijer and Kelly Hunt and Khandan Keyomarsi}, journal={Cell cycle}, year={2009}, volume={8 7}, pages={1062-8} }