Low levels of DNA sequence variation among adapted genotypes of hexaploid wheat

  title={Low levels of DNA sequence variation among adapted genotypes of hexaploid wheat},
  author={Glenn J Bryan and Pauline Stephenson and Andrew J. Collins and Jane Kirby and Jeffrey B. Smith and Mike D. Gale},
  journal={Theoretical and Applied Genetics},
 PCR products from regions corresponding to sequences hybridising to wheat RFLP probes were sequenced in order to establish the level of DNA sequence variation among adapted wheat genotypes. Hexaploid bread wheat shows a very low rate of nucleotide polymorphism, approximately 1 polymorphic nucleotide per 1000 basepairs. Differences in PCR product length can be exploited to design genome-specific amplicons, which may have use in gene tagging or in diagnostic applications. Interpretation of… CONTINUE READING
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