Low-force DNA condensation and discontinuous high-force decondensation reveal a loop-stabilizing function of the protein Fis.
@article{Skoko2005LowforceDC, title={Low-force DNA condensation and discontinuous high-force decondensation reveal a loop-stabilizing function of the protein Fis.}, author={Dunja Skoko and J. Yan and R. Johnson and J. Marko}, journal={Physical review letters}, year={2005}, volume={95 20}, pages={ 208101 } }
We report single-DNA-stretching experiments showing that the protein Fis, an abundant bacterial chromosome protein of E. coli, mediates a dramatic DNA condensation to zero length. This condensation occurs abruptly when DNA tension is reduced below a protein-concentration-dependent threshold f* < 1 pN. Following condensation, reopening under larger forces proceeds via a series of discrete jumps, indicating that Fis is able to stabilize DNA crossings. Our experiments suggest that Fis may play a… CONTINUE READING
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