Low-dose three-dimensional hard x-ray imaging of bacterial cells

Abstract

We have imaged the three-dimensional density distribution of unstained and unsliced, freeze-dried cells of the gram-positive bacterium Deinococcus radiodurans by tomographic x-ray propagation microscopy, i.e. projection tomography with phase contrast formation by free space propagation. The work extends previous x-ray imaging of biological cells in the simple in-line holography geometry to full three-dimensional reconstruction, based on a fast iterative phase reconstruction algorithm which circumvents the usual twin-image problem. The sample is illuminated by the highly curved wave fronts emitted from a virtual quasi-point source with 10 nm cross section, realized by two crossed x-ray waveguides. The experimental scheme allows for a particularly dose efficient determination of the 3D density distribution in the cellular structure.

DOI: 10.1186/2192-2853-1-10

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Cite this paper

@article{Bartels2012LowdoseTH, title={Low-dose three-dimensional hard x-ray imaging of bacterial cells}, author={Matthias Bartels and Marius Priebe and Robin Niklas Wilke and Sven P Kr{\"{u}ger and Klaus Giewekemeyer and Sebastian Kalbfleisch and Christian Olendrowitz and Michael Sprung and Tim Salditt}, journal={Optical Nanoscopy}, year={2012}, volume={1}, pages={1-7} }