Low dose and gene gun immunization with a hepatitis C virus nonstructural (NS) 3 DNA-based vaccine containing NS4A inhibit NS3/4A-expressing tumors in vivo

@article{Frelin2003LowDA,
  title={Low dose and gene gun immunization with a hepatitis C virus nonstructural (NS) 3 DNA-based vaccine containing NS4A inhibit NS3/4A-expressing tumors in vivo},
  author={Lars Frelin and Mats Alheim and A-m Chen and Jonas S{\"o}derholm and Bj{\"o}rn Rozell and Christina Barnfield and Peter Liljestr{\"o}m and Matti S{\"a}llberg},
  journal={Gene Therapy},
  year={2003},
  volume={10},
  pages={686-699}
}
The hepatitis C virus (HCV) protease and helicase encompasses the nonstructural (NS) 3 protein and the cofactor NS4A, which targets the NS3/4A-complex to intracellular membranes. We here evaluate the importance of NS4A in NS3-based genetic immunogens. A full-length genotype 1 NS3/4A gene was cloned into a eucaryotic expression vector in the form of NS3/4A and NS3 alone. Transient transfections revealed that the inclusion of NS4A increased the expression levels of NS3. Subsequently, immunization… CONTINUE READING

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References

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Characterization of a monoclonal antibody and its single-chain antibody fragment recognizing the nucleoside triphosphatase/helicase domain of the hepatitis C virus nonstructural 3 protein

ZX Zhang
Clin Diagn Lab Immunol • 2000

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