Low-cost serum-free medium for the BTI-Tn5B1-4 insect cell line.

@article{Donaldson1998LowcostSM,
  title={Low-cost serum-free medium for the BTI-Tn5B1-4 insect cell line.},
  author={Margaret Donaldson and Michael Louis Shuler},
  journal={Biotechnology progress},
  year={1998},
  volume={14 4},
  pages={573-9}
}
The BTI-Tn5B1-4 insect cell line, commercially available as the High Five cell line (Invitrogen), supports higher levels of recombinant protein production compared to existing insect cell lines. Proprietary serum-free media such as ExCell 405 (JRH Biosciences), Express Five (Life Technologies), IS BAC (Irvine Scientific), and CCM3 (HyClone) are available which were developed specifically for a suspension culture of High Five cells. While these media are highly optimized, a lower cost… CONTINUE READING

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The amino acid and carbohydrate metabolism of the Tn5B1 - 4 cells was first examined .
The amino acid and carbohydrate metabolism of the Tn5B1 - 4 cells was first examined .
It was found that asparagine was nearly depleted during batch growth in Ex - Cell 405 , without limitations in glutamine , other amino acids , or glucose .
Amino AcidsChemical structure ofGlutamic Acid
It was found that asparagine was nearly depleted during batch growth in Ex - Cell 405 , without limitations in glutamine , other amino acids , or glucose .
It was found that asparagine was nearly depleted during batch growth in Ex - Cell 405 , without limitations in glutamine , other amino acids , or glucose .
While these media are highly optimized , a lower cost alternative is desirable for large - scale protein production which is also serum - free and supports good cell growth ( > 5 x 10(6 ) cells / mL ) and recombinant protein production ( > 50 mg / L of secreted protein ) .
While these media are highly optimized , a lower cost alternative is desirable for large - scale protein production which is also serum - free and supports good cell growth ( > 5 x 10(6 ) cells / mL ) and recombinant protein production ( > 50 mg / L of secreted protein ) .
It was found that asparagine was nearly depleted during batch growth in Ex - Cell 405 , without limitations in glutamine , other amino acids , or glucose .
Supplementation with additional aspargine in a 1.5% lipid - Pluronic F-68 mixture did not improve growth , suggesting that a lipid was growth - limiting and not an amino acid .
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