Low-cost media formulation for culture of brain tumor spheroids (neurospheres).

@article{Monterey2013LowcostMF,
  title={Low-cost media formulation for culture of brain tumor spheroids (neurospheres).},
  author={Michael D. Monterey and Nicholas J. Szerlip and Saroj P. Mathupala},
  journal={BioTechniques},
  year={2013},
  volume={55 2},
  pages={83-8}
}
Recent studies have found that the biological features of primary tumors are faithfully recapitulated when a patient's tumor is processed and then maintained as a 3-D spheroid in specialized cell culture media. However, a major drawback for maintenance and routine passage of primary tumors as spheroids has been the high cost of custom-formulated media compared to regular serum-supplemented media. Here we report the formulation of a cost-effective, serum-free medium in which high-grade primary… CONTINUE READING