Low-cost generation of Good Manufacturing Practice-grade CD19-specific chimeric antigen receptor-expressing T cells using piggyBac gene transfer and patient-derived materials.

@article{Ramanayake2015LowcostGO,
  title={Low-cost generation of Good Manufacturing Practice-grade CD19-specific chimeric antigen receptor-expressing T cells using piggyBac gene transfer and patient-derived materials.},
  author={Saumya Ramanayake and I Bilmon and David C. Bishop and Ming-Celine Dubosq and Emily Blyth and Leighton E. Clancy and David J Gottlieb and Kenneth P. Micklethwaite},
  journal={Cytotherapy},
  year={2015},
  volume={17 9},
  pages={
          1251-67
        }
}
BACKGROUND AIMS Protocols for the production of CD19-specific chimeric antigen receptor (CAR19) T cells are often complex and expensive because of the use of retroviral and lentiviral vectors or the need for CAR19 T-cell enrichment. We aimed to simplify the generation of CAR19 T cells from the peripheral blood of normal donors and patients using the piggyBac transposon system of gene modification. METHODS We varied electroporation voltage, cytokines and stimulation conditions for the… CONTINUE READING

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Enhanced Expression of Anti-CD19 Chimeric Antigen Receptor in piggyBac Transposon-Engineered T Cells

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