Low-cost, low-input RNA-seq protocols perform nearly as well as high-input protocols

@inproceedings{Combs2015LowcostLR,
  title={Low-cost, low-input RNA-seq protocols perform nearly as well as high-input protocols},
  author={Peter A. Combs and Michael B. Eisen},
  booktitle={PeerJ},
  year={2015}
}
Recently, a number of protocols extending RNA-sequencing to the single-cell regime have been published. However, we were concerned that the additional steps to deal with such minute quantities of input sample would introduce serious biases that would make analysis of the data using existing approaches invalid. In this study, we performed a critical evaluation of several of these low-volume RNA-seq protocols, and found that they performed slightly less well in per-gene linearity of response, but… CONTINUE READING
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