Low-affinity CD4+ T cells are major responders in the primary immune response

@inproceedings{Martinez2016LowaffinityCT,
  title={Low-affinity CD4+ T cells are major responders in the primary immune response},
  author={Ryan J. Martinez and Rakieb Andargachew and Hunter A. Martinez and Brian D Evavold},
  booktitle={Nature communications},
  year={2016}
}
A robust primary immune response has been correlated with the precursor number of antigen-specific T cells, as identified using peptide MHCII tetramers. However, these tetramers identify only the highest-affinity T cells. Here we show the entire CD4+ T-cell repertoire, inclusive of low-affinity T cells missed by tetramers, using a T-cell receptor (TCR) signalling reporter and micropipette assay to quantify naive precursors and expanded populations. In vivo limiting dilution assays reveal… CONTINUE READING
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