Low access resistance perforated patch recordings using amphotericin B

@article{Rae1991LowAR,
  title={Low access resistance perforated patch recordings using amphotericin B},
  author={James L. Rae and Kim D. Cooper and Peter Stuart Gates and Mitchell Aaron Watsky},
  journal={Journal of Neuroscience Methods},
  year={1991},
  volume={37},
  pages={15-26}
}
We present experimental procedures describing the creation of perforated patches by use of amphotericin B. In 13 different cellular preparations, access resistances below 10 M omega were achieved and with blunt electrode tips, access resistances of 3-4 M omega were possible. In addition to using the techniques to measure whole cell currents, we have used them to measure single channel currents in a new "outside-out patch" preparation and we have utilized them to measure the resting voltage of… CONTINUE READING

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