Low 13C-background for NMR-based studies of ligand binding using 13C-depleted glucose as carbon source for microbial growth: 13C-labeled glucose and 13C-forskolin binding to the galactose-H+ symport protein GalP in Escherichia coli.

@article{Patching2004Low1F,
  title={Low 13C-background for NMR-based studies of ligand binding using 13C-depleted glucose as carbon source for microbial growth: 13C-labeled glucose and 13C-forskolin binding to the galactose-H+ symport protein GalP in Escherichia coli.},
  author={Simon G Patching and Richard B Herbert and John O'reilly and Adrian R Brough and Peter J F Henderson},
  journal={Journal of the American Chemical Society},
  year={2004},
  volume={126 1},
  pages={86-7}
}
Obtrusive 13C-backgrounds can be a problem in 13C NMR-based studies of ligand binding to bacterial membrane transport proteins in their natural state in inner membranes. This is largely solved for the bacterial galactose-H+ symport protein GalP by growing the producing organism Escherichia coli on 13C-depleted glucose (13C </= 0.07%) as the main carbon source. 13C solid-state NMR-based binding studies for the inhibitor forskolin 1 and the transported substrate glucose 2, both singly labeled… CONTINUE READING