Loss of the penicillinase plasmid after treatment of Staphylococcus aureus with sodium dodecyl sulfate.

@article{Sonstein1972LossOT,
  title={Loss of the penicillinase plasmid after treatment of Staphylococcus aureus with sodium dodecyl sulfate.},
  author={S A Sonstein and Jeffrey N Baldwin},
  journal={Journal of bacteriology},
  year={1972},
  volume={109 1},
  pages={262-5}
}
Two strains of Staphylococcus aureus (PC1 and 196E), when grown in medium containing 0.002% sodium dodecyl sulfate (SDS), showed from 96.1 to 100% loss of the ability to produce penicillinase. Resistance to cadmium and zinc was lost concomitantly with the ability to produce penicillinase. A comparison of the rate of curing by SDS with the curing effects exerted by elevated temperature and ethidium bromide suggested that SDS is a more effective plasmid curing agent for susceptible strains of S… CONTINUE READING