Loss-of-function mutations at the rim of the funnel of mechanosensitive channel MscL.

@article{Yoshimura2004LossoffunctionMA,
  title={Loss-of-function mutations at the rim of the funnel of mechanosensitive channel MscL.},
  author={Kenjiro Yoshimura and Takeshi Nomura and Masahiro Sokabe},
  journal={Biophysical journal},
  year={2004},
  volume={86 4},
  pages={2113-20}
}
MscL is a bacterial mechanosensitive channel that is activated directly by membrane stretch. Although the gene has been cloned and the crystal structure of the closed channel has been defined, how membrane tension causes conformational changes in MscL remains largely unknown. To identify the site where MscL senses membrane tension, we examined the function of the mutants generated by random and scanning mutagenesis. In vitro (patch-clamp) and in vivo (hypoosmotic-shock) experiments showed that… CONTINUE READING

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