Loss of free amino groups in the water-insoluble fraction of nuclear senile cataracts.

@article{GarcaCastieiras1983LossOF,
  title={Loss of free amino groups in the water-insoluble fraction of nuclear senile cataracts.},
  author={Sixto Garc{\'i}a-Casti{\~n}eiras and M N Miranda-Rivera},
  journal={Investigative ophthalmology & visual science},
  year={1983},
  volume={24 9},
  pages={1181-7}
}
Free amino groups were analyzed in the water-insoluble fraction (WIF) of cataractous human lenses of the nuclear sclerosis, pigmented type. Two modified versions of the trinitrobenzene sulfonic acid (TNBS) procedure for the determination of free amino groups in proteins (AFSA Habeeb, Anal Biochem 14:328-336, 1966) were used for this purpose. The… CONTINUE READING