Loss of detectability of Charcot-Leyden crystal protein transcripts in blood cells after treatment with dimethyl sulfoxide.

@article{Fll2008LossOD,
  title={Loss of detectability of Charcot-Leyden crystal protein transcripts in blood cells after treatment with dimethyl sulfoxide.},
  author={J. F{\"o}ll and Ines Volkmer and Corinna Giersberg and Malte Kornhuber and Gerd Horneff and Martin Sebastian Staege},
  journal={Journal of immunological methods},
  year={2008},
  volume={339 1},
  pages={99-103}
}
Charcot-Leyden crystal protein (CLC) is a major secretory effector protein of eosinophils. In addition, CLC has been identified as marker for regulatory T-cells and differential expression of CLC has been described under diverse pathological conditions. By analysis of DNA microarray data from peripheral blood mononuclear cells (PBMC) we found differences for the expression of CLC between PBMC that had been cryopreserved or had been used for RNA isolation immediately after cell separation… CONTINUE READING
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Analysis of relative gene expression data using real - time quantitative PCR and the 2 ( - Delta Delta C ( T ) ) Method

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