Loose coupling between chemical and mechanical reactions in actomyosin energy transduction.

In order to study the mechanochemical coupling in actomyosin energy transduction, the sliding distance of an actin filament induced by a myosin head during one ATP hydrolysis cycle was obtained using an in vitro movement assay, which permitted quantitative and simultaneous measurements of (1) the movements of single fluorescently-labeled actin filaments on… CONTINUE READING