Corpus ID: 86779122

Loop-mediated Isothermal Amplification (LAMP) 법을 이용한 백묵병 원인균 Ascosphera apis의 검출법 개발

@inproceedings{2011LoopmediatedIA,
  title={Loop-mediated Isothermal Amplification (LAMP) 법을 이용한 백묵병 원인균 Ascosphera apis의 검출법 개발},
  author={이보람 and 유미선 and Nguyen Van Phu and 노지나 and 윤병수},
  year={2011}
}
Chalkbrood disease of honeybee is caused by a apecies of fungus, Ascosphaera apis (A. apis), which could affect larvae of honeybee. In this study, new LAMP method for the specific detection of A. apis in honeybee was developed (A. apis-LAMP). This method is amplified DNA with high specificity, efficiency and rapidity under isothermal conditions. Four A. apis-specific primers named A. apis-F3/B3/FIP/BIP were designed based on A. apis 18S ribosomal RNA gene (GenBank, M83264.1). After optimization… Expand