Looking for reference genes for real-time quantitative PCR experiments in Rhodnius prolixus (Hemiptera: Reduviidae).

@article{Majerowicz2011LookingFR,
  title={Looking for reference genes for real-time quantitative PCR experiments in Rhodnius prolixus (Hemiptera: Reduviidae).},
  author={David Majerowicz and Michele Alves-Bezerra and Raquel Logullo and Andr{\'e} Luiz Fonseca-de-Souza and Jos{\'e} Roberto Meyer-Fernandes and Gl{\'o}ria Regina Cardoso Braz and Katia Calp Gondim},
  journal={Insect molecular biology},
  year={2011},
  volume={20 6},
  pages={713-22}
}
Quantitative real-time PCR (qPCR) has become one of the most used techniques to measure gene expression. However, normalization of gene expression data against reference genes is essential, although these are usually used without any kind of validation. The expression of seven genes was compared in organs of Rhodnius prolixus under diverse conditions, using published software to test gene expression stability. Rp18S and elongation factor 1 (RpEF -1) were the most reliable genes for… CONTINUE READING
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