Looking Inside Adenovirus
@article{Harrison2010LookingIA, title={Looking Inside Adenovirus}, author={Stephen C. Harrison}, journal={Science}, year={2010}, volume={329}, pages={1026 - 1027} }
Vaccines and gene therapy vectors could benefit from new insights into structure. Adenoviruses are a large group of DNA viruses with a distinguished experimental history, including contributions to the discovery of RNA splicing and the elucidation of central pathways in cell transformation (1). Some adenoviruses are human pathogens. They are also very much in vogue as potential vaccine and gene therapy vectors. Structure-based redesign of their cell-targeting and antigenic properties is…
29 Citations
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References
SHOWING 1-10 OF 12 REFERENCES
Crystal Structure of Human Adenovirus at 3.5 Å Resolution
- Biology, ChemistryScience
- 2010
The x-ray structure at 3.5 angstrom resolution of the 150-megadalton adenovirus capsid containing nearly 1 million amino acids is reported, and interactions between the major capsid protein (hexon) and several accessory molecules that stabilize the capsid are described.
Atomic Structure of Human Adenovirus by Cryo-EM Reveals Interactions Among Protein Networks
- BiologyScience
- 2010
The structure of the whole human adenovirus virion is reported at 3.6 angstroms resolution by cryo–electron microscopy (cryo-EM), revealing in situ atomic models of three minor capsid proteins, extensions of the major capsids, and interactions within three protein-protein networks.
Structural and Phylogenetic Analysis of Adenovirus Hexons by Use of High-Resolution X-Ray Crystallographic, Molecular Modeling, and Sequence-Based Methods
- BiologyJournal of Virology
- 2003
The combination of crystallography, molecular modeling, and phylogenetic analysis defines a conserved molecular core that can serve as the armature for the directed design of novel hexons.
Hexon-chimaeric adenovirus serotype 5 vectors circumvent pre-existing anti-vector immunity
- BiologyNature
- 2006
Recombinant, replication-incompetent adenovirus serotype 5 (rAd5) vector-based vaccines for human immunodeficiency virus type 1 and other pathogens have proved highly immunogenic in preclinical studies but will probably be limited by the high prevalence of pre-existing anti-Ad5 immunity in human populations, particularly in the developing world.
Subunit interactions in bovine papillomavirus
- BiologyProceedings of the National Academy of Sciences
- 2010
It should be possible to obtain image reconstructions of comparable resolution from cryoEM images of asymmetric particles, and it is estimated that such a reconstruction will require about 1.5 million images to achieve the same number of averaged asymmetric units; structural variability will increase this number substantially.
3.88 Å structure of cytoplasmic polyhedrosis virus by cryo-electron microscopy
- BiologyNature
- 2008
The polyhedrin-binding domain, a structure that has potential in nanobiotechnology applications, is identified and observed a helix-to-β-hairpin conformational change between the two conformational states of the capsid shell protein in the region directly interacting with genomic RNA.
Crystal structure of the receptor-binding domain of adenovirus type 5 fiber protein at 1.7 A resolution.
- ChemistryStructure
- 1994
A triple β-spiral in the adenovirus fibre shaft reveals a new structural motif for a fibrous protein
- BiologyNature
- 1999
The structure reveals a novel triple β-spiral fibrous fold for the shaft of the adenovirus type 2 fibre shaft plus the receptor-binding head domain, which has implications for folding of fibrous proteins and for the design of a new class of artificial, silk-like fibrous materials.
Visualization of α-Helices in a 6-Ångstrom Resolution Cryoelectron Microscopy Structure of Adenovirus Allows Refinement of Capsid Protein Assignments
- ChemistryJournal of Virology
- 2006
The structure of adenovirus was determined to a resolution of 6 Å by cryoelectron microscopy (cryoEM) single-particle image reconstruction and secondary structure predictions for the additional capsid proteins that currently lack atomic resolution structures were analyzed.
Near-atomic resolution using electron cryomicroscopy and single-particle reconstruction
- ChemistryProceedings of the National Academy of Sciences
- 2008
A reconstruction, obtained by using recently developed image processing methods, of the rotavirus inner capsid particle (“double-layer particle” or DLP) at a resolution suitable for interpretation by an atomic model is presented, establishing single-particle reconstruction as a high-resolution technique.