Looking Inside Adenovirus

@article{Harrison2010LookingIA,
  title={Looking Inside Adenovirus},
  author={S. Harrison},
  journal={Science},
  year={2010},
  volume={329},
  pages={1026 - 1027}
}
Vaccines and gene therapy vectors could benefit from new insights into structure. Adenoviruses are a large group of DNA viruses with a distinguished experimental history, including contributions to the discovery of RNA splicing and the elucidation of central pathways in cell transformation (1). Some adenoviruses are human pathogens. They are also very much in vogue as potential vaccine and gene therapy vectors. Structure-based redesign of their cell-targeting and antigenic properties is… Expand
View on AAAS
ncbi.nlm.nih.gov
30 Citations
Highly Influential Citations
1
Background Citations
12
Methods Citations
2

30 Citations

Citation Type

Citation Type

Atomic Structures of Minor Proteins VI and VII in Human Adenovirus
TLDR
The improved structure of human Ad5 unambiguously confirms previous cryo-EM models of proteins IIIa, VIII, and IX and explains the likely cause of conflict in the crystallography models, and reveals atomic details of membrane-lytic protein VI and genome-condensing protein VII. Expand
The E3 ubiquitin ligase Mind bomb 1 enhances nuclear import of viral DNA by inactivating a virion linchpin protein that suppresses exposure of virion pathogen-associated molecular patterns
TLDR
The data uncover how the ubiquitin-proteasome system controls the function of a virion linchpin protein suppressing pathogen-associated molecular patterns and triggers viral DNA uncoating at the nuclear pore complex for nuclear import and infection. Expand
Structure of viruses: a short history
  • M. Rossmann
  • Biology, Medicine
  • Quarterly Reviews of Biophysics
  • 2013
TLDR
This review is a partially personal account of the discovery of virus structure and its implication for virus function, covering all aspects of structural virology and to acknowledge relevant individuals. Expand
Adenoviruses Types, Cell Receptors and Local Innate Cytokines in Adenovirus Infection
TLDR
This review discusses the innate immune response primarily during wild-type adenovirus infection because this serves as the basis for understanding the response during both natural infection and exposure to adanovirus vectors. Expand
Expanding the Spectrum of Adenoviral Vectors for Cancer Therapy
TLDR
A state-of-the-art overview of currently performed and completed clinic trials with OAs and an adenovirus library is provided, providing novel possibilities for developing innovativeadenoviral vectors for cancer treatment. Expand
A Novel Role for PX, a Structural Protein of Fowl Adenovirus Serotype 4 (FAdV4), as an Apoptosis-Inducer in Leghorn Male Hepatocellular Cell
TLDR
It is reported here that FAdV4 infection caused apoptosis in Leghorn male hepatocellular (LMH) cells and that PX, a structural protein of F adenovirus Serotype 4, acted as a major viral factor inducing apoptosis. Expand
Icosahedral virus structures and the protein data bank
TLDR
The growth in size and complexity of icosahedral viruses from the first early studies of small RNA plant viruses and human picornaviruses up to the larger and more complex bacterial phage, insect, and human disease viruses such as Zika, hepatitis B, Adeno and Polyoma virus is described. Expand
Baculovirus-expressed FAdV-4 penton base protein protects chicken against hepatitis-hydropericardium syndrome
TLDR
Baculovirus-expressed penton base protein is a promising candidate subunit vaccine for Hepatitis-hydropericardium syndrome and is shown to provide 100% protection to the chickens. Expand
Designer DNA architecture offers precise and multivalent spatial pattern-recognition for viral sensing and inhibition
TLDR
This work demonstrates a designer DNA nanostructure that can act as a template to display multiple binding motifs with precise spatial pattern-recognition properties, and shows that this approach can confer exceptional sensing and potent viral inhibitory capabilities. Expand
Ultra scale‐down approaches to study the centrifugal harvest for viral vaccine production
TLDR
The results indicate that virus infected cells do not actually show any increase in sensitivity to shear, and may indeed become less shear sensitive, in a similar manner to that previously observed in old or dead cell cultures. Expand
...
1
2
3
...

References

SHOWING 1-10 OF 13 REFERENCES
Crystal Structure of Human Adenovirus at 3.5 Å Resolution
TLDR
The x-ray structure at 3.5 angstrom resolution of the 150-megadalton adenovirus capsid containing nearly 1 million amino acids is reported, and interactions between the major capsid protein (hexon) and several accessory molecules that stabilize the capsid are described. Expand
Structural and Phylogenetic Analysis of Adenovirus Hexons by Use of High-Resolution X-Ray Crystallographic, Molecular Modeling, and Sequence-Based Methods
TLDR
The combination of crystallography, molecular modeling, and phylogenetic analysis defines a conserved molecular core that can serve as the armature for the directed design of novel hexons. Expand
Hexon-chimaeric adenovirus serotype 5 vectors circumvent pre-existing anti-vector immunity
TLDR
Recombinant, replication-incompetent adenovirus serotype 5 (rAd5) vector-based vaccines for human immunodeficiency virus type 1 and other pathogens have proved highly immunogenic in preclinical studies but will probably be limited by the high prevalence of pre-existing anti-Ad5 immunity in human populations, particularly in the developing world. Expand
The structure of the human adenovirus 2 penton.
TLDR
The complex of penton base with bound N-terminal fiber peptide, determined at 3.5 A resolution, shows that the universal fiber motif FNPVYPY binds at the interface of adjacent pentin base monomers and results in a localized structural rearrangement in the insertion domain of the pentonbase. Expand
Subunit interactions in bovine papillomavirus
TLDR
It should be possible to obtain image reconstructions of comparable resolution from cryoEM images of asymmetric particles, and it is estimated that such a reconstruction will require about 1.5 million images to achieve the same number of averaged asymmetric units; structural variability will increase this number substantially. Expand
3.88 Å structure of cytoplasmic polyhedrosis virus by cryo-electron microscopy
TLDR
The polyhedrin-binding domain, a structure that has potential in nanobiotechnology applications, is identified and observed a helix-to-β-hairpin conformational change between the two conformational states of the capsid shell protein in the region directly interacting with genomic RNA. Expand
Crystal structure of the receptor-binding domain of adenovirus type 5 fiber protein at 1.7 A resolution.
TLDR
The large buried surface area and numerous polar interactions in the trimer indicate that this form of the knob protein is predominant in solution, suggesting a possible assembly pathway for the native fiber protein. Expand
A triple β-spiral in the adenovirus fibre shaft reveals a new structural motif for a fibrous protein
TLDR
The structure reveals a novel triple β-spiral fibrous fold for the shaft of the adenovirus type 2 fibre shaft plus the receptor-binding head domain, which has implications for folding of fibrous proteins and for the design of a new class of artificial, silk-like fibrous materials. Expand
Visualization of α-Helices in a 6-Ångstrom Resolution Cryoelectron Microscopy Structure of Adenovirus Allows Refinement of Capsid Protein Assignments
TLDR
The structure of adenovirus was determined to a resolution of 6 Å by cryoelectron microscopy (cryoEM) single-particle image reconstruction and secondary structure predictions for the additional capsid proteins that currently lack atomic resolution structures were analyzed. Expand
Near-atomic resolution using electron cryomicroscopy and single-particle reconstruction
TLDR
A reconstruction, obtained by using recently developed image processing methods, of the rotavirus inner capsid particle (“double-layer particle” or DLP) at a resolution suitable for interpretation by an atomic model is presented, establishing single-particle reconstruction as a high-resolution technique. Expand
...
1
2
...