Long-term shoot regeneration from pampas grass (Cortaderia selloana Schult.) through manipulation of growth regulators in vitro
@article{Robacker2004LongtermSR, title={Long-term shoot regeneration from pampas grass (Cortaderia selloana Schult.) through manipulation of growth regulators in vitro}, author={Carol D. Robacker}, journal={Plant Cell Reports}, year={2004}, volume={14}, pages={689-693} }
A regeneration system for ‘Pumila’ pampas grass (Cortaderia selloana Schult.) that yields plants over many months and allows control of morphogenesis was developed. Immature inflorescence explants cultured for three 4-week passages on MS basal medium supplemented with 4.5 μM 2,4-D and 8.9 μM BA yielded a dark green callus that organized into shoot primordia. Rate of shoot development was increased after transfer of shoot primordia to medium supplemented with 9.8 μM IBA. Subculture every 4–6…
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