Long-term self-renewal of human pluripotent stem cells on human recombinant laminin-511

@article{Rodin2010LongtermSO,
  title={Long-term self-renewal of human pluripotent stem cells on human recombinant laminin-511},
  author={Sergey Rodin and Anna Domogatskaya and Susanne Str{\"o}m and Emil M. Hansson and Kenneth R Chien and Jose Inzunza and Outi Hovatta and Karl Tryggvason},
  journal={Nature Biotechnology},
  year={2010},
  volume={28},
  pages={611-615}
}
We describe a system for culturing human embryonic stem (hES) cells and induced pluripotent stem (iPS) cells on a recombinant form of human laminin-511, a component of the natural hES cell niche. The system is devoid of animal products and feeder cells and contains only one undefined component, human albumin. The hES cells self-renewed with normal karyotype for at least 4 months (20 passages), after which the cells could produce teratomas containing cell lineages of all three germ layers. When… Expand
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References

SHOWING 1-10 OF 33 REFERENCES
A culture system using human foreskin fibroblasts as feeder cells allows production of human embryonic stem cells.
TLDR
Post-natal, commercially available human foreskin fibroblasts are used as feeder cells for derivation and continued undifferentiated growth of hES cells, convenient for IVF units, because no fetal human tissues or tissue from operations are needed. Expand
Derivation of Human Embryonic Stem Cell Lines in Serum Replacement Medium Using Postnatal Human Fibroblasts as Feeder Cells
TLDR
It is concluded that hESC lines can be successfully derived using SR medium and postnatal human fibroblasts as feeder cells, a step toward xeno‐free conditions and facilitates the use of these cells in transplantation. Expand
Feeder-independent culture of human embryonic stem cells
TLDR
Modifications to the medium (mTeSR1) that include the use of animal-sourced proteins (bovine serum albumin (BSA) and Matrigel) and cloned zebrafish basic fibroblast growth factor (zbFGF) are described. Expand
Embryonic stem cell lines from human blastocysts: somatic differentiation in vitro
TLDR
The derivation of pluripotent embryonic stem (ES) cells from human blastocysts is described, providing a model to study early human embryology, an investigational tool for discovery of novel growth factors and medicines, and a potential source of cells for use in transplantation therapy. Expand
Human embryonic stem cells derived without feeder cells
TLDR
A new stem-cell line was derived from human embryos under completely cell and serum free conditions, eliminating exposure of human embryonic stem cells and their progeny to animal and human feeder layers, and thus the risk of contamination with pathogenic agents capable of transmitting diseases to patients. Expand
Mechanical isolation of the inner cell mass is effective in derivation of new human embryonic stem cell lines.
TLDR
Mechanical isolation of the ICM proved to be an effective way to derive new hESC lines and the xeno-components of immunosurgery could be avoided. Expand
Recombinant human laminin isoforms can support the undifferentiated growth of human embryonic stem cells.
TLDR
Results show that rhLM-111, -332, and -511 are good substrates to expand undifferentiated hESCs due to their high affinity to integrin alpha6beta1 expressed on hESC. Expand
Identification of the critical extracellular matrix proteins that promote human embryonic stem cell assembly.
TLDR
The goal of these studies was to determine the key extracellular matrix (ECM) components that regulate assembly and growth of hESC and reveal for the first time the crucial role of the ECM proteins laminin-511 and nidogen-1 in h ESC assembly. Expand
A high-efficiency system for the generation and study of human induced pluripotent stem cells.
TLDR
This work sought to create a doxycycline-inducible lentiviral system to convert primary human fibroblasts and keratinocytes into human induced pluripotent stem cells (hiPSCs), and developed a strategy to induce hiPSC formation at high frequency. Expand
Laminin‐511 but Not ‐332, ‐111, or ‐411 Enables Mouse Embryonic Stem Cell Self‐Renewal In Vitro
TLDR
The results suggest that recombinant laminin isoforms can provide a basis for defined surface coating systems for feeder‐free maintenance of undifferentiated mammalian ES cells in vitro. Expand
...
1
2
3
4
...