Long-term self-renewal of human pluripotent stem cells on human recombinant laminin-511

@article{Rodin2010LongtermSO,
  title={Long-term self-renewal of human pluripotent stem cells on human recombinant laminin-511},
  author={Sergey N. Rodin and Anna Domogatskaya and Susanne Str{\"o}m and Emil M. Hansson and Kenneth R. Chien and Jos{\'e} Antonio Inzunza and Outi Hovatta and Karl Tryggvason},
  journal={Nature Biotechnology},
  year={2010},
  volume={28},
  pages={611-615}
}
We describe a system for culturing human embryonic stem (hES) cells and induced pluripotent stem (iPS) cells on a recombinant form of human laminin-511, a component of the natural hES cell niche. The system is devoid of animal products and feeder cells and contains only one undefined component, human albumin. The hES cells self-renewed with normal karyotype for at least 4 months (20 passages), after which the cells could produce teratomas containing cell lineages of all three germ layers. When… CONTINUE READING
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