Long-term preservation of mouse spermatozoa after freeze-drying and freezing without cryoprotection.

@article{Ward2003LongtermPO,
  title={Long-term preservation of mouse spermatozoa after freeze-drying and freezing without cryoprotection.},
  author={Monika A. Ward and Takehito Kaneko and Hirokazu Kusakabe and John D. Biggers and David G Whittingham and Ryuzo Yanagimachi},
  journal={Biology of reproduction},
  year={2003},
  volume={69 6},
  pages={2100-8}
}
The widespread production of mice with transgenes, disrupted genes and mutant genes, has strained the resources available for maintaining these mouse lines as live populations, and dependable methods for gamete and embryo preservation in these lines are needed. Here we report the results of intracytoplasmic sperm injection (ICSI) with spermatozoa freeze-dried or frozen without a cryoprotectant after storage for periods up to 1.5 years. Freeze-dried samples were stored at 4 degrees C. Samples… CONTINUE READING