Long-term performance and stability of molecular shotgun lipidomic analysis of human plasma samples.

@article{Heiskanen2013LongtermPA,
  title={Long-term performance and stability of molecular shotgun lipidomic analysis of human plasma samples.},
  author={Laura A Heiskanen and Matti Suoniemi and Hung Xuan Ta and Kirill Tarasov and Kim Ekroos},
  journal={Analytical chemistry},
  year={2013},
  volume={85 18},
  pages={8757-63}
}
The stability of the lipid concentration levels in shotgun lipidomics analysis was tracked over a period of 3.5 years. Concentration levels in several lipid classes, such as phospholipids, were determined in human plasma lipid extracts. Impact of the following factors on the analysis was investigated: sample amount, internal standard amount, and sample dilution factor. Moreover, the reproducibility of lipid profiles obtained in both polarity modes was evaluated. Total number of samples analyzed… CONTINUE READING

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