Long-term passage of tachyzoites in tissue culture can attenuate virulence of Neospora caninum in vivo.

@article{Bartley2006LongtermPO,
  title={Long-term passage of tachyzoites in tissue culture can attenuate virulence of Neospora caninum in vivo.},
  author={Paul M Bartley and Stephen I Wright and J. Boira Sales and Francesca Chianini and David Buxton and Elisabeth Ann. Innes},
  journal={Parasitology},
  year={2006},
  volume={133 Pt 4},
  pages={
          421-32
        }
}
To determine whether prolonged in vitro passage would result in attenuation of virulence in vivo, Neospora caninum tachyzoites were passaged for different lengths of time in vitro and compared for their ability to cause disease in mice. Groups of Balb/c mice were inoculated intraperitoneally with 5 x 10(6) or 1 x 10(7) of low-passage or high-passage N. caninum tachyzoites. The mice were monitored for changes in their demeanour and body weight, and were culled when severe clinical symptoms of… CONTINUE READING
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