Long-term organ culture of adult rat colon.

  title={Long-term organ culture of adult rat colon.},
  author={Abulkalam M. Shamsuddin and L. A. Barrett and Herman Autrup and Curtis C. Harris and Benjamin F. Trump},
  journal={Pathology, research and practice},
  volume={163 4},
Colon explants from adult rats were maintained in culture for over 3 months in our laboratories with good epithelial preservation and cellular differentiation. The light and transmission electron microscopic features of rat colon mucosa during the culture period are described. In all the explants that remained viable, there was an initial phase of degeneration of the surface and crypt cells, later these areas were repopulated in one week, showing well-formed crypts, goblet cells, and… CONTINUE READING