Long-term monolayer culture of human anterior pituitary cells: functional validation of a method.

@article{RodgersNeame1984LongtermMC,
  title={Long-term monolayer culture of human anterior pituitary cells: functional validation of a method.},
  author={N T Rodgers-Neame and Edwin L. Bradley and Richard E. Blackwell},
  journal={Fertility and sterility},
  year={1984},
  volume={41 1},
  pages={
          75-80
        }
}
This study demonstrates the response of human pituitary cell monolayers to a wide variety of hormonal stimuli. Appropriate release of luteinizing hormone (LH) and prolactin (PRL) was used as a verification of cell function. Cells that had been in culture for 20 days, with no hormonal additions, were exposed to LH-releasing hormone (LH-RH) continuously for 14 days. This resulted in an immediate fivefold increase in secretion of LH followed by a depression in LH production over the remaining 10… CONTINUE READING

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