Long-term maintenance of mature pulmonary parenchyma cultured in serum-free conditions.

@article{Siminski1992LongtermMO,
  title={Long-term maintenance of mature pulmonary parenchyma cultured in serum-free conditions.},
  author={J T Siminski and Terrance J Kavanagh and Emil Y. Chi and Ganesh Raghu},
  journal={The American journal of physiology},
  year={1992},
  volume={262 1 Pt 1},
  pages={L105-10}
}
Current concepts of the pathogenesis of lung injury and repair are derived from in vitro cellular and in vivo investigations. Studies with viable ex vivo models may offer additional insights into disease processes, since essential cellular interactions would be maintained. However, a major limiting factor has been the availability of a model that maintains normal parenchymal structure, viability, and homeostasis beyond 4 wk in serum-free conditions. We have succeeded in establishing an ex vivo… CONTINUE READING