Long-term maintenance of low concentrations of fructose for the study of hepatic glucose phosphorylation.

@article{Phillips1999LongtermMO,
  title={Long-term maintenance of low concentrations of fructose for the study of hepatic glucose phosphorylation.},
  author={John W. Phillips and Michael N Berry},
  journal={The Biochemical journal},
  year={1999},
  volume={337 ( Pt 3)},
  pages={497-501}
}
The stimulation of glucose phosphorylation in isolated hepatocytes by low fructose concentrations is transient due to the rapid metabolism of fructose. To prolong this stimulatory effect fructose was enzymically generated in the incubation medium from either sucrose with invertase or inulin with inulinase. A maximal rate of glucose phosphorylation was achieved when fructose was formed at at least 0.01 micromol/min, which maintained a concentration of 70 microM fructose in the medium. In the… CONTINUE READING