Long-term influence of bone marrow-derived mesenchymal stem cells on liver ischemia-reperfusion injury in a rat model.


BACKGROUND The aim of this study was to evaluate the long-term usefulness of intraportal injection of the bone marrow-derived mesenchymal stem cells (BM-MSCs) in limitation of experimentally induced ischemia-reperfusion injury (IRI) in a rat model. MATERIAL AND METHODS Twenty Wistar rats were divided into 3 groups: donor group (n=5), study group (n=10), and control group (n=5). IRI was performed using a modified hanging-weight system after left portal triad occlusion in study group animals. Isolated autologous BM-MSCs were labeled with fluorochrome PKH-26 then intraportally injected into the rats in the study group. Control group animals were intraportally injected with 1 ml of PBS. Follow-up was 3 months, after which animals were sacrificed for histopathological examination. Migration of BM-MSCs into different organs was examined. RESULTS H&E staining of liver tissue sections from "time zero" biopsies did not show many irregularities in structural or histological construction compared to liver sections from the control group. However, a small amount of centrilobular hepatocyte necrosis and coagulative necrosis with neutrophil infiltration areas was observed in liver sections of the study group. The migration assay of BM-MSCs labeled with PKH-26 showed the highest positive BM-MSCs staining (6%) in the spleen, while few positively stained cells were found (2%) in liver sections. No BM-MSCs were detected in brain, kidney, or lung tissues. CONCLUSIONS These results suggest that intraportal bone marrow-derived mesenchymal stem cell injection is safe and cells do not migrate chaotically to other organs after targeted implementation.

DOI: 10.12659/AOT.892364

Cite this paper

@article{Nowacki2015LongtermIO, title={Long-term influence of bone marrow-derived mesenchymal stem cells on liver ischemia-reperfusion injury in a rat model.}, author={Maciej Nowacki and Łukasz Nazarewski and Marta Pokrywczyńska and Tomasz Kloskowski and Dominik Tyloch and Katarzyna Pietkun and Arkadiusz Jundziłł and Marta Rasmus and Karolina Warda and Maciej Gagat and Alina Grzanka and Magdalena Bodnar and Andrzej Marszałek and Marek Krawczyk and Samy L Habib and Tomasz Adam Drewa}, journal={Annals of transplantation}, year={2015}, volume={20}, pages={132-40} }