Long-term cultures of testicular biopsies from boys with cryptorchidism: effect of FSH and LH on the number of germ cells.

@article{Larsen2002LongtermCO,
  title={Long-term cultures of testicular biopsies from boys with cryptorchidism: effect of FSH and LH on the number of germ cells.},
  author={Hans-Peter Ejler Larsen and J\orgen Mogens Thorup and Lene Theil Skovgaard and Dina Cortes and Anne Grete Sjolte Byskov},
  journal={Human reproduction},
  year={2002},
  volume={17 2},
  pages={383-9}
}
BACKGROUND A long-term culture system of testicular biopsies from boys with undescended testes was established to evaluate the effect of gonadotrophins on germ cell survival and growth. METHODS Biopsies from 25 boys with cryptorchidism, aged 1.1-9.9 years, were obtained at the time of surgery. Each biopsy was subdivided into fragments and cultured for 1 or 3 weeks in a testis culture medium without gonadotrophins or supplemented with FSH (50 IU/l) or LH (5 IU/l), or a combination of FSH (50… CONTINUE READING