Long term culture of mouse hybridoma HB8852 cells in a protein free medium

Abstract

We developed a protein free medium based on ERDF medium containing 80 μM FeSO4, ethanolamine and selenite. Although this medium contained neither transferrin nor insulin, the medium could support long term culture of mouse hybridoma HB8852 without any significant changes in antibody production. 
DOI: 10.1007/BF01086327

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Cite this paper

@article{Shinmoto2005LongTC, title={Long term culture of mouse hybridoma HB8852 cells in a protein free medium}, author={Hiroshi Shinmoto and Shun'ichi Dosako and Shin'ichi Taneya}, journal={Biotechnology Letters}, year={2005}, volume={13}, pages={683-686} }