Long-term culture and passage of human fetal liver cells that synthesize albumin

  title={Long-term culture and passage of human fetal liver cells that synthesize albumin},
  author={Mary Ann Sells and Jonathon Chernoff and Arturo Cerda and Charles Bowers and David A. Shafritz and Nathan Kase and Judith K. Christman and George Acs},
  journal={In Vitro Cellular & Developmental Biology},
Long-term cultures of hepatocytes were established from livers of human fetuses obtained by abortion at 18 to 23 wk of gestation. Cells obtained by collagenase dissociation of liver were maintained in defined serum-free medium on a substratum of positively charged plastic. Under these conditions, the cells divide and form a confluent monolayer. After multiple passages over a period of 3 mo., the cells retained an epithelioid morphology and continued to synthesize and secrete albumin. 

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