Long-term culture and functional characterization of follicular cells from adult normal human thyroids.

@article{Curcio1994LongtermCA,
  title={Long-term culture and functional characterization of follicular cells from adult normal human thyroids.},
  author={Francesco Curcio and Francesco Saverio Ambesi-Impiombato and Giuseppina Perrella and H G Coon},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={1994},
  volume={91 19},
  pages={9004-8}
}
We have obtained long-term cultures of differentiated proliferating follicular cells from normal adult human thyroid glands. In vitro growth of such human cells has been sustained by a modified F-12 medium, supplemented with bovine hypothalamus and pituitary extracts and no added thyrotropin. Cultures have been expanded, cloned, frozen, successfully retrieved, and characterized. Functional characterization of these cells shows constitutive thyroglobulin production and release and thyrotropin… CONTINUE READING