Long-term cultivation of human osteoblasts.

  title={Long-term cultivation of human osteoblasts.},
  author={Ronny Schmidt and Klaus D. Kulbe},
  journal={Bone and mineral},
  volume={20 3},
Primary cultures of osteoblastic cells were obtained from human bone tissue after corrective surgery. Osteoblasts were isolated with a combined enzymatic and cell migration method and characterized by a high expression of alkaline phosphatase (AP), the marker enzyme for osteoblasts. For quantification of AP a new sensitive method has been developed. Also the secretion of osteocalcin measured by radioimmunoassay (RIA) which was stimulated by calcitriol, characterized isolated cells as… CONTINUE READING


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