Long-term, high-resolution imaging in the mouse neocortex through a chronic cranial window

  title={Long-term, high-resolution imaging in the mouse neocortex through a chronic cranial window},
  author={Anthony Holtmaat and Tobias Bonhoeffer and David D. K. Chow and Jyoti A. Chuckowree and Vincenzo De Paola and Sonja B. Hofer and Mark H{\"u}bener and Tara Keck and Graham Knott and Wei-Chung Allen Lee and Ricardo Mostany and Tom D Mrsic-Flogel and Elly Nedivi and Carlos Portera-Cailliau and Karel Svoboda and Joshua T. Trachtenberg and Linda Wilbrecht},
  journal={Nature Protocols},
To understand the cellular and circuit mechanisms of experience-dependent plasticity, neurons and their synapses need to be studied in the intact brain over extended periods of time. Two-photon excitation laser scanning microscopy (2PLSM), together with expression of fluorescent proteins, enables high-resolution imaging of neuronal structure in vivo. In this protocol we describe a chronic cranial window to obtain optical access to the mouse cerebral cortex for long-term imaging. A small bone… CONTINUE READING
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