Long-read DNA metabarcoding of ribosomal RNA in the analysis of fungi from aquatic environments.

@article{Heeger2018LongreadDM,
  title={Long-read DNA metabarcoding of ribosomal RNA in the analysis of fungi from aquatic environments.},
  author={Felix Heeger and Elizabeth C Bourne and Christiane Baschien and Andrey M Yurkov and Boyke Bunk and Cathrin Spr{\"o}er and J{\"o}rg Overmann and Camila J. Mazzoni and Michael T Monaghan},
  journal={Molecular ecology resources},
  year={2018},
  volume={18 6},
  pages={
          1500-1514
        }
}
  • Felix Heeger, Elizabeth C Bourne, +6 authors Michael T Monaghan
  • Published in Molecular ecology resources 2018
  • Medicine, Biology
  • DNA metabarcoding is widely used to study prokaryotic and eukaryotic microbial diversity. Technological constraints limit most studies to marker lengths below 600 base pairs (bp). Longer sequencing reads of several thousand bp are now possible with third-generation sequencing. Increased marker lengths provide greater taxonomic resolution and allow for phylogenetic methods of classification, but longer reads may be subject to higher rates of sequencing error and chimera formation. In addition… CONTINUE READING

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