Long-range quantitative PCR for determining inactivation of adenovirus 2 by ultraviolet light.

Abstract

AIMS An extra-long-range quantitative PCR (LR-qPCR) method was developed for estimating genome damage to adenovirus 2 caused by UV irradiation. The objective was to use LR-qPCR as a rapid method to determine adenovirus UV inactivation. METHODS The LR-qPCR consisted of two steps: a long-range PCR (up to 10 kb fragment) and a real-time, quantitative (q) PCR… (More)
DOI: 10.1111/jam.12169

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