Long-range chromosomal engineering is more efficient in vitro than in vitro

@article{Olson2005LongrangeCE,
  title={Long-range chromosomal engineering is more efficient in vitro than in vitro},
  author={Lisa E. Olson and Jason Tien and Sarah South and Roger H Reeves},
  journal={Transgenic Research},
  year={2005},
  volume={14},
  pages={325-332}
}
Cre/LoxP mediated chromosomal engineering in embryonic stem (ES) cells has a variety of applications, including the creation of model systems for studying aneuploidy. Targeted meiotic recombination (TAMERE) was proposed as a high efficiency in vivo alternative to effect Cre-mediated recombination, in which Cre recombinase under control of the Synaptonemal Complex 1 promoter is expressed during male meiosis in transgenic mice. TAMERE has been successfully used with LoxP sites up to 100 kb apart… CONTINUE READING