Long-Term Primary Culture of Highly-Pure Rat Embryonic Hippocampal Neurons of Low-Density

@article{Yang2010LongTermPC,
  title={Long-Term Primary Culture of Highly-Pure Rat Embryonic Hippocampal Neurons of Low-Density},
  author={Hao Yang and R. Cong and Luo Na and G. Ju and S. You},
  journal={Neurochemical Research},
  year={2010},
  volume={35},
  pages={1333-1342}
}
  • Hao Yang, R. Cong, +2 authors S. You
  • Published 2010
  • Biology, Medicine
  • Neurochemical Research
  • In order to develop a simplified method for long-term primary culture of highly-pure rat embryonic hippocampal neurons of low-density (103 cells/cm2), we optimized and modified conventional culturing methods. The modifications of our simplified method include: (1) combinational application of two growth substrates, tail collagen and poly-L-lysine, to coat plastic culture dishes and coverslips for a better neuronal attachment; (2) dissociation of hippocampal tissues with combinational use of two… CONTINUE READING

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    References

    Publications referenced by this paper.
    SHOWING 1-10 OF 36 REFERENCES
    Culturing nerve cells
    • 1,135
    COLLAGEN SUBSTRATA FOR STUDIES ON CELL BEHAVIOR
    • 1,456
    • PDF
    Isolation and Culture of Post-Natal Mouse Cerebellar Granule Neuron Progenitor Cells and Neurons
    • 61
    • PDF
    Induction of a neurite-promoting factor in rat brain following injury or deafferentation
    • 169
    Growth of dissociated neurons in culture dishes coated with synthetic polymeric amines
    • 67
    Optimized and efficient preparation of astrocyte cultures from rat spinal cord
    • 15
    Metabolism and functions of glutathione in brain
    • 1,428
    • PDF