Long-Term Primary Culture of Highly-Pure Rat Embryonic Hippocampal Neurons of Low-Density

@article{Yang2010LongTermPC,
  title={Long-Term Primary Culture of Highly-Pure Rat Embryonic Hippocampal Neurons of Low-Density},
  author={Hao Yang and Rui Cong and Luo Na and Gong Ju and Si-wei You},
  journal={Neurochemical Research},
  year={2010},
  volume={35},
  pages={1333-1342}
}
In order to develop a simplified method for long-term primary culture of highly-pure rat embryonic hippocampal neurons of low-density (103 cells/cm2), we optimized and modified conventional culturing methods. The modifications of our simplified method include: (1) combinational application of two growth substrates, tail collagen and poly-L-lysine, to coat plastic culture dishes and coverslips for a better neuronal attachment; (2) dissociation of hippocampal tissues with combinational use of two… CONTINUE READING

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