Long-Chain Acyl CoA Regulation of Protein Kinase C and Fatty Acid Potentiation of Glucose-Stimulated Insulin Secretion in Clonalβ -Cells.

@article{Yaney2000LongChainAC,
  title={Long-Chain Acyl CoA Regulation of Protein Kinase C and Fatty Acid Potentiation of Glucose-Stimulated Insulin Secretion in Clonalβ -Cells.},
  author={Gordon C. Yaney and Helen M. Korchak and Barbara E Corkey},
  journal={Endocrinology},
  year={2000},
  volume={141 6},
  pages={1989-1998}
}
Pancreatic β-cells contain protein kinase C (PKC) isoforms that may play a role in insulin secretion. Activity of PKC classes (cPKC, nPKC, aPKC) and their regulation by acyl-CoA derivatives was examined in extracts of clonal pancreatic β-cells (HIT) by protein phosphorylation. PKC classes were distinguished based on their previously defined cofactor requirements. Down-regulation of PKC by phorbol esters was confirmed by Western blotting and resulted in the complete loss of cPKC activity… CONTINUE READING

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